In <i>Vitro</i> Clonal Propagation from Adult Material of a Savannah Species of Socio-Economic Importance: <i>Annona senegalensis</i> Pers.

Annona senegalensis (Pers.) or Annone from Senegal is a species found in the West African savannah and para-littoral sands. It offers real possibilities of socio-economic use, particularly medical, nutritional, ecosystem conservation poverty alleviation fields. However, this wild remained not fully exploited despite its potentialities. As result, there need to regenerate species; therefore, vitro propagation adult material was undertaken. For purpose, axillary nodes plant regrowth taken elderly subjects were aseptically introduced into different media enriched with phytohormones. Among 3 culture tested, that Murashige Skoog (MS, 1962) most reactive made it possible develop micropropagation protocol for A. senegalensis. Thus, introduction these explants containing cytokinins (BAP Kinetin) significantly increased reactivity compared without hormones. If best average numbers shoots (2.3) (5.3) are obtained MS + BAP 2 mg·L−1, 91.66%, addition 0.1 mg·L−1 NAA gave length (8.25 cm) vitroplants. An induction time days darkness IBA at 50 followed by transfer hormone-free expression medium (MS/2) under light, resulted rooting rate 58.33%. After gradual weaning young plants mini-greenhouse, shade weeks, 50% survived. This regeneration can therefore be adopted clonal improved trying other hormonal combinations based on auxins considering ex-vitro during weaning.